Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 12;7:46040. doi: 10.1038/srep46040

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

The standard protocol of the Illumina V3 MiSeq 2 × 300 kit was changed from a bidirectional to a unidirectional format (Extended Unidirectional Sequencing, EUS). The panels above indicate the proportion of EUS reads passing filter in the forward (red line) or reverse (blue line) directions only, and show the percentage consensus agreement (orange line) at each base position for: (a) the best example profile for rbcL (Rhagodia spinesens X98); (b) the worst example profile for rbcL (Eremophila scoparia M56B). Inset shows base-by-base consensus agreement in boxed area.