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. 2017 Apr 12;7:46381. doi: 10.1038/srep46381

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A schematic drawing is presented showing antral follicle development in non-ovulating rats and the first two cycles after the first ovulation, as well as the corresponding Pub-scores from −5 to +8. The age of the animals is matched to the day of the first ovulation (PND-40 in the example; encircled). An ovarian section (stained with hematoxylin and eosin) showing two CL generations, those of the current cycle (two days of age; metestrus; CLm) and regressing CL (CLR), together with eggs at the isthmus (blue arrow in the inset) and class F3 follicles. In accordance, a Pub-score of +6 is assigned and the first ovulation occurred at PND-35 (red box).