Fig. 2.

Cytotoxicity and potency of lycorine against an epidemic strain of WNV. (A) Cytotoxicity of lycorine in Vero cells. Cytotoxicity was examined by incubation of Vero cells with the indicated concentrations of lycorine. Cell viability was measured by an MTT assay, and is presented as a percentage of colorimetric absorbance derived from the compound-treated cells compared with that from the mock-treated cells (with 1% DMSO). Average results from three experiments are shown. (B) Inhibition of WNV infection in cell culture. Vero cells were infected with an epidemic strain of WNV (0.1 MOI). The infected cells were immediately treated with lycorine at the indicated concentrations. Viral titers in culture fluids at 42 h p.i. were determined by plaque assays.