TABLE 1.
Study strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference(s) |
|---|---|---|
| S. aureus strains | ||
| NCTC 8325-4 | Wild-type strain cured of known prophages, rsbU mutant | 27, 37 |
| RN4220 | NCTC 8325-4 r-; capable of stably maintaining recombinant plasmids | 26 |
| SA-K2069 | RN4220 norA::lacZ | This study |
| SH1000 | rsbU+ derivative of NCTC 8325-4 | 15 |
| SA-K2124 | SH1000 norA::lacZ | This study |
| SA-K2173 | SA-K2124 IR mutant | This study |
| SA-K2161, -K2291, -K2293 | SA-K2124 flqB (T→A, C, or G, respectively) | This study |
| SA-K2242 | SA-K2124 TTAATT mutant | This study |
| ISP794 | NCTC 8325 pig-131; rsbU mutant | 40 |
| SA-K2382 | ISP794 norA::lacZ | Q.-C. Truong-Boldoc and D. C. Hooper |
| SA-K2383 | SA-K2382 flqB (T→A) | Q.-C. Truong-Boldoc and D. C. Hooper |
| Newman | Easily transducible clinical strain; rsbU+ | 7 |
| SA-K2083 | Newman norA::lacZ | This study |
| SA-K2159 | SA-K2083 flqB (T→A) | This study |
| Plasmids | ||
| pAZ106 | Suicide vector for construction of lacZ fusions, Emr | 24 |
| pK304 | pAZ106 containing 5′ internal norA fragment | This study |
| pTS2tetK | Temperature-sensitive vector used for allele replacement, Tcr | J. Higgins and T. Foster |
| pCU1 | S. aureus-E. coli shuttle vector, Cmr | 4 |
| pTL2989 | pCL52.2 containing mgrA (P1+P2) cloned between EcoRI and HindIII sites | C. Y. Lee |
| pK404 | pCU1 containing EcoRI-HindIII fragment of pTL2989 (mgrA [P1+P2]) | This study |
| pALC2073 | S. aureus-E. coli shuttle vector containing a tetracycline-inducible promoter controlling expression of cloned genes, Cmr | 6 |
| pK410 | pALC2073 containing mgrA under control of its tetracycline-inducible promoter | This study |
a
TTAATT, putative consensus sequences to which a norA regulatory protein(s) binds; Emr, Tcr, Cmr, erythromycin, tetracycline, and chloramphenicol resistance selection; P1+P2, both promoters of mgrA present.