TABLE 2.

Primers used in the study

Primer	Positiona	Sequence (5′-3′)b
abc2Fc	−309, −288	+GGCAATCGCTTGTGTTTTAGCG
abc2R	+1735, +1713	−GTGAATCCCATGATGTTGATACC
abc3Fd	+108, +129	+GATTGGCCGCAATGGCCGTGGG
abc3R	+1123, +1101	−GGTGAGCCAAAGTGGCTTCGCC
abc3F2	+1349, +1372	+GATGAACCCCTTAATTACTTGG
abc7F	−203, −182	+GACGAAAAGAGGGATTCGTGCG
abc7R	−11, −32	−CCATAAAGCAAAATTGATGCAG
abc2Fe	−309, −288	+GGCAATCGCTTGTGTTTTAGCG
Isa mut	−105, −85	−GAATTCTTTAAGCTAATTTC
Mega primer F (OG1) (double stranded)f	−309, −85	GGCAATCGCTTGTGTTTTAGCGAATAATTTAAATTTTTATCAGAATGAAAGCAAGGGGCCTTCGTTGACAAATCAGTGAAGAGATGCTAAATTCTAGATAATAAAAGACGAAAAGAGGGATTCGTGCGTTGAAAATTTAAGTTAATTTTTTAGAGAAACTGTATAACTTTGCCTGATTGTAACGGGTGGAGTGTGCGGATTTTCGGAAATTAGCTTAAAGAATTC
Mega primer F (V583) (double stranded)g	−309, −85	GGCAATCGCTTGTGTTTTAGCGAATAATTTAAATTTTTATCAGAATGAAAGCAAGGGGCCTTCGTTGACAAATCAGTGAAGAGATGCTAAATTCTAGATAATAAAAGACGAAAAGAGGGATTCGTGCGTTGAAAATTTAAGTTAATTTTTTAGAGAAACTGTATAACTTTGCCTGAATATAACGGGTGGAGTGTGCGGATTTTCGGAAATTAGCTTAAAGAATTC

^aBases relative to ATG.

^b+, sense primer; −, antisense primer.

^cPrimers abc2F and abc2R were used to amplify intact lsa on a 2-kb fragment.

^dPrimers abc3F to abc7R are intragenic primers used to confirm the lsa sequence.

^ePrimers abc2F and lsa mut were used for site-directed mutagenesis (to generate mega primer).

^fMega primer F(OG1) and abc2R were used to generate lsa_V-M. Boldfaced T and G were targeted in site-directed mutagenesis.

^gMega primer F(V583) and abc2R were used to generate lsa_OG-M. Boldfaced A and A were targeted in site-directed mutagenesis.