TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	DNA sequencea
PnPy F2	CCBGGCRHRMMVGACGARCCCCAGCA
PnPy R1	CCSCCGRYRSWKTCRTGSRCVCCGTTGTYGA
Pepmut F1	GCCBVTBVTYKTBGAYGGHGACACSGGR
Pepmut R2	CGGCVCGSAKTHKGTGRTTSGCSYARAT
phsA FOR	ACCCGTACATGGCGTTACTC
phsA REV	CTCCAGATCTCACCCACCTG
phsB 5′ end FOR	AGGGTCAGTACTCGGTGTGG
phsB 5′ end REV	CGAAGTGGATGGTGTAGGTG
pmi FOR	ACGTCCGGATGAACTACCTC
pmi REV	CTACGGAGGGGAATTCAGGT
pepmut FOR	GCTGAAGAACCTGCTGCAC
pepmut REV	TGTAGCGGTTCTCGTCCTG
pat FOR	CCGGGGACGACTTCTTCT
pat REV	GCCGTGCAGGTACAGCAG GGCGCGCCATCGATGGTGCACTTTAGGTG
oriR6K/HK attB FOR	AAAAAGGTTGAGTCGCTAACTGTCAGCCCGCC
oriR6K REV	GGCGCGCCATCGATAATTCACTGGG GGCAATTC
F5-9G, downstream	TACCGTCTGCTATGGTCCTCCGTCTCCT
KO F	GTGGGTGAGTGTAGGCTGGAGCTGCTTCG
F5-9G, downstream	GAGAATTCGCGGCCGCATAATACGACT
KO R	CACTATAGGTTCCGGGGATCCGTCGACCTG
RS1 check F	AACACTTAACGGCTGACATGG
RS1 check R	GAGTAGCCGCTTTCAAATGG
8BT7F	GCGGTACTGTCATGGATGC
8BT7R	CTAGTCCGGCCAGCAGTTC
8BT3F	CCGTCGAGGTTGAAGGAGTA
8BT3R	GATGAAGACCTACGCCAAGC
Mu-Seq L1	CGATAAGCGCCTCTGTTCCT
Mu-Seq R1	GGACTCTGGGGTTCGAAATG
Mu-Seq R5	TCTATCGCCTTCTTGACGAG
pAH144 DEL P1	GTAGGTCATTATTAGTCAAAATAAAATCATTTG TCGATTTCAATTTTGTCTGTGTAGGCTGGAGCTGC TTCG
pAH144 DEL P2	TGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTC CCTACTCTCGCATGGGATTCCGGGGATCCGTCG ACC
ori-BglII	CGCGCGAGATCTAATTCTGTCAGCCGTTAAG
bla-BamHI	CGCGCGGATCCTCTAGAGGCGCGCAGGTGG
MuR-A/E/B	GGCGCGCCGAATTCAGATCTGAAGCGGCGCACGAAAA ACG
MuR-A/M	GGCGCGCCACGCGTGAAGCGGCGCACGAAAAACG
Supercos-aphF	GGCGCGCCGGATCCTTGGCAGAACATATCCATCG
Supercos-aphR	GGCGCGCCGGATCCTCATTTCGAACCCCAGAGTC
Tsr-L	GGCGCGCCTCTAGACGGTGATCAAGGCGAATACT
Tsr-R	GGCGCGCCGCGGCCGCTCCGAGGAACAGAGGCGCTT

^aStandard abbreviations are used: R = A or G, Y = C or T, M = A or C, K = G or T, S = C or G, W = A or T, H = A or C or T, B = C or G or T, V = A or C or G, and D = A or G or T.