TABLE 3.
Summary of fosmid 5-9G open reading frames
| ORF | No. of amino acids | Protein homologya (Swiss-Prot accession no.) | % Amino acid identityb |
|---|---|---|---|
| phpA | 69 | S. viridochromogenes putative protein Orf1 (Q9KWY9) | 98.5 |
| Pseudomonas aeruginosa putative MbtH-like protein (Q9I169) | 51.5 | ||
| phsB | 1,189 | S. viridochromogenes peptide synthetase III (Q9KWY8) | 99.8 |
| phpB | 553 | S. viridochromogenes putative protein OrfM (Q8KLJ4) | 100 |
| Myxococcus xanthus putative membrane protein (Q9S433) | 35.9 | ||
| phsC | 1,086 | S. viridochromogenes peptide synthetase II (Q9K WY7) | 99.5 |
| pmi | 894 | S. viridochromogenes phosphinomethylmalate isomerase (AcnP) (Q9RIL3) | 99.8 |
| phpC | 395 | Amycolatopsis orientalis putative iron-dependent alcohol dehydrogenase (Q9XBE8) | 33.1 |
| phpD | 431 | No significant homology | N/A |
| phpE | 336 | Methanopyrus kandleri phosphoglycerate dehydrogenase homolog (Q8TYK0) | 39 |
| ppm | 313 | S. viridochromogenes phosphoenolpyruvate phosphomutase (O86937) | 96.8 |
| S. hygroscopicus phosphoenolpyruvate phosphomutase (BcpB) (P29247) | 83.3 | ||
| ppd | 397 | S. viridochromogenes phosphonopyruvate decarboxylase (O86938) | 100 |
| S. hygroscopicus phosphonopyruvate decarboxylase (BcpC) (O54271) | 84.3 | ||
| phpF | 184 | S. viridochromogenes putative protein OrfX (O86939) | 100 |
| S. hygroscopicus putative protein OrfX (Q9LCB1) | 91.1 | ||
| Pyrococcus furiosus nicotinamide-nucleotide adenylyltransferase (Q8U3K8) | 33.7 | ||
| phpG | 419 | S. hygroscopicus OrfZZ (364 aa) (Q9LCB3) | 84.2 |
| Aeropyrum pernix 2,3-BPGi phosphoglycerate mutase homolog (Q9YBI2) | 38.4 | ||
| phpH | 398 | S. hygroscopicus carboxyphosphonopyruvate synthase (BcpE, 333 aa) (Q54274) | 82.9 |
| Pseudomonas syringae pv. tomato enolase 1 homolog (Q886M3) | 38.9 | ||
| phpI | 296 | S. hygroscopicus carboxyphosphonopyruvate phosphonomutase (BcpA) (P11435) | 95.5 |
| phpJ | 466 | S. hygroscopicus unnamed PTT cluster protein (416 aa) (Q54272) | 90.1 |
| Sinorhizobium meliloti putative aldehyde dehydrogenase (Q92UV7) | 44.4 | ||
| phpK | 549 | S. hygroscopicus N-acetyldemethylphosphinothricin tripeptide P-methylase (BcpD) (Q54273) | 92.1 |
| pms | 440 | S. viridochromogenes phosphinomethylmalate synthase fragment (205 aa) (Q65169) | 100 |
| S. hygroscopicus phosphinomethymalate synthase (PmmS) (Q9LCB4) | 91.3 | ||
| phsA | 622 | S. viridochromogenes peptide synthetase 1 (Q56170) | 99.1 |
| pat | 183 | S. viridochromogenes demethylphosphinothricin N-acetyltransferase (Q57146) | 100 |
| S. hygroscopicus bialaphos resistance protein (Bar) (P16426) | 84.6 | ||
| dea | 299 | S. viridochromogenes N-acetylphosphinothricin tripeptide deacetylase (Q56171) | 99.3 |
| S. hygroscopicus bialaphos acetylhydrolase (Bah) (Q01109) | 87.8 | ||
| phpL | 253 | S. hygroscopicus orf1 gene product (Q03093) | 82.4 |
| Streptomyces coelicolor thioesterase II (Q9LAS9) | 37.6 | ||
| phpM | 260 | S. hygroscopicus orf2 gene product (Q03094) | 81 |
| Streptomyces avermitilis thioesterase (Q93H55) | 45.3 | ||
| phpN | 477 | S. hygroscopicus orf3 gene product (Q03095) | 74.6 |
| S. coelicolor putative transmembrane transport protein (Q9XAH4) | 60.4 | ||
| phpR | 261 | S. hygroscopicus bialaphos regulatory protein (BrpA) (Q01108) | 63.9 |
| orf416 | 416 | S. coelicolor putative transmembrane protein (Q9L223) | 51.7 |
| orf192 | 192 | Bacillus halodurans putative DNA-binding protein (Q9K8U3) | 33.7 |
| orf261 | 261 | Mycobacterium paratuberculosis putative formamidopyrimidine-DNA glycoslylase (AAS03645) | 54.2 |
| orf321 | 321 | S. coelicolor putative integral membrane protein (Q9RJN5) | 67.6 |
| orf290 | 290 | S. avermitilis putative acyl coenzyme A thioesterase (Q82MT5) | 94 |
| orf571′ | 571 | S. coelicolor putative helicase carboxyl terminus fragment (571 aa) (Q9KZJ3) | 94.7 |
a
aa, amino acids; 2,3-BPGi, bisphosphoglycerate independent.
b
Percent identity and closest homologs were based on FASTA searches conducted 21 May 2004. N/A, not available.