FIG. 3.

PlyGBS activity against GBS in vitro. GBS NCTC 11237 cells were resuspended in 40 mM phosphate buffer (pH 5.0) and mixed in 96-well microtiter plates with 40 U of PlyGBS. The OD₆₀₀ (solid triangles) was monitored by a spectrophotometer. To check the cell viability (solid squares), the cells were centrifuged, diluted, and plated on THY agar plates for enumeration. Control experiments (open circles) with the addition of phosphate buffer (pH 5.0) were performed under the same conditions.