Fig.6.

Chop^–/– mice are significantly protected from the palmitate-enriched diet-induced increase in BACE1 expression and ensuing Aβ genesis. A) Representative western blots show that nine-month-old Chop^–/– mice fed a palmitate-enriched diet for three months, do not exhibit the increase in BACE1 protein levels as well as the accompanying increase in sAβPPβ and CTFβ levels concomitant with a decrease in sAβPPα and CTFα levels, to the same degree in the hippocampal region compared to the C57BL/6J wild-type mice fed a palmitate-enriched diet. B, C) Chop^–/– mice fed a palmitate-enriched diet do not exhibit the increase in BACE1 mRNA expression (B) and BACE1 activity (C), to the same degree in the hippocampal region compared to the C57BL/6J wild-type mice fed a palmitate-enriched diet. D) ELISA immunoassays show that the Chop^–/– mice fed a palmitate-enriched diet have significantly lower levels of total formic acid-soluble Aβ_1 - 40 and Aβ_1 - 42 species in the hippocampus, compared to the C57BL/6J wild-type mice fed a palmitate-enriched diet. Data is expressed as Mean±S.D and includes determination made in six (n = 6) different animals from each group. ^*p < 0.05, ^***p < 0.001 versus C57BL/6J wild-type mice fed a control chow diet; ^†p < 0.05, ^††p < 0.01, versus C57BL/6J wild-type mice fed a palmitate-enriched diet; ^Δp < 0.05 versus Chop^–/– mice fed a control chow diet. PA, palmitic acid.