Table 5.
Antibody | Dilution | Amount | Host | Manufacturer | Catalog # |
ATF3 | 1:500 | 10 μg | rabbit | Sigma Aldrich | HPA001562 |
ATF4 | 1:1000 | 5 μg | rabbit | Cell Signaling Technology | 11815 (D4B8) |
ATF6 | 1:1000 | 5 μg | rabbit | Active Motif | 40962 |
β-Actin | 1:2500 | 2 μg | mouse | Santa Cruz BioTechnology | sc-47778 (C4) |
BACE1 | 1:1000 | 5 μg | rabbit | EMD Millipore | AB5832 |
CHOP | 1:500 | 10 μg | rabbit | Cell Signaling Technology | 5554 (D46F1) |
CTFα/ CTFβ | 1:400 | 12.5 μg | rabbit | BioLegend | 825001 |
Histone H3 | 1:1000 | 5 μg | rabbit | Santa Cruz BioTechnology | sc-8654 (C16) |
p-Ser724 IRE1α | 1:200 | 25 μg | rabbit | Abcam | ab48187 |
IRE1α | 1:500 | 10 μg | rabbit | Cell Signaling Technology | 3294 (14C10) |
p-Thr980 PERK | 1:500 | 10 μg | rabbit | Cell Signaling Technology | 3179 (16F8) |
PERK | 1:500 | 10 μg | rabbit | Cell Signaling Technology | 3192 (C33E10) |
sAβPPα | 1:500 | 10 μg | rabbit | BioLegend | 813501 |
sAβPPβ | 1:500 | 10 μg | rabbit | BioLegend | 813401 |