Fig. 9.

DOE improves the survival rates of mice treated with endotoxin through blockade of HMGB1 release. a BALB/c mice (n = 8 per group) were treated with LPS (10 mg/kg, i.p.) and then injected with a single dose of DOE (20 mg/kg, i.p.) 0, 3, 6, and 9 h later. Motality was monitored for up to 2 weeks every day. b BALB/c mice (n = 4 per group) were treated with a single dose of DOE (20 mg/kg, i.p.) with or without a lethal injection of endotoxin (LPS, 10 mg/kg, i.p.). Circulating levels of HMGB1 were detected by immunoblot analysis of sera collected at 20 h post-LPS injection. As a loading control, Ponceau S staining was used. c Schematic representation of DOE-mediated inhibition of HMGB1 release. ^#, p < 0.01 vs LPS-treated group