Fig. 3.

Bright field images ORO staining of different histological grades of fatty liver degeneration. a Grad 0: normal liver, no fat deposition visible. b Grade 1: Positive red signal intracytoplasmatically in hepatocytes around central vein. c Grade 2: half of hepatocytes of a liver lobule show clear red stained fatty vacuoles. The edge to an area with only finely red stained lipid droplets is marked with arrows. d Grade 3: entire liver lobule shows red stained, clear demarcated fatty vacuoles in hepatocytes