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. 2017 Apr 12;8:623. doi: 10.3389/fmicb.2017.00623

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Copyright © 2017 da Costa, Resende, Santos, Zoccal, Faccioli, Kipnis and Junqueira-Kipnis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-1α production by lung homogenates from Bacillus Calmette–Guérin (BCG) vaccinated mice. Mice were intranasally vaccinated with BCG-pLA71 or rBCG-CMX. The animals were euthanized 4 days after immunization, and the production of IL-1α was evaluated in lung homogenates. BALB/c mice (A); C57BL/6 mice (B). ^∗p < 0.05 difference between the rBCG-CMX or BCG-pLA71 group and the saline group. No difference was observed between the BCG-pLA71 and rBCG-CMX groups. A total of five mice were used per group. The experiments were repeated three times.