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. 2017 Apr 12;8:623. doi: 10.3389/fmicb.2017.00623

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Copyright © 2017 da Costa, Resende, Santos, Zoccal, Faccioli, Kipnis and Junqueira-Kipnis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Macrophage infected by rBCG-CMX vaccine increases MHC-II. Bone marrow-derived macrophages were infected with BCG or rBCG-CMX (MOI 5) for 3 h. After this time, excess non-phagocytosed bacteria were removed. After 24 h of culture, the macrophages were analyzed by flow cytometry. F4/80 positive cells were evaluated for the mean intensity of fluorescence (MFI) of MHC-II expression. ^∗Difference between the rBCG-CMX group and the saline group. ^#Difference between the rBCG-CMX group and the BCG group, p < 0.05. The experiments were repeated three times in quadruplicate.