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Production of cytokines by BMMs from C57BL/6 mice stimulated with rAg85c and rCMX proteins. Macrophages derived from the bone marrow of C57BL/6 mice were cultured with 20 μg/mL recombinant proteins for 24 h. After this period, the collected supernatant was subjected to analyses of IL-6 (A), IL-1α (B), and TGF-β (C) cytokines. ∗p < 0.05 difference between stimuli and the medium. The experiments were repeated three times in quadruplicate.
