TABLE 5.
Effects of BM0 and BM2 on growth, viability, and Pma1p activity for S. cerevisiae and fungal pathogens
| Species and strain | MIC90/MFCa (μM)
|
IC50 (μM) for Pma1p ATPase
|
Concn (μM)/time (h) to >98% cell deathb
|
|||||
|---|---|---|---|---|---|---|---|---|
| BM0
|
BM2
|
BM0 (pH 7.0) | BM2 (pH 7.0) | BM0 (pH 7.0) | BM2 (pH 7.0) | |||
| pH 7.0 | pH 7.3 (7.5)c | pH 7.0 | pH 7.3 (7.5)c | |||||
| S. cerevisiae | ||||||||
| T48 | 1.3s | 0.63s/2.5c | 5s | 0.63s/2.5c | 2.5 | 0.5 | 100/17.5 | 20/17.5 |
| AD/PDR5+ | 10c | 2.5c | 5c | 1.25c | ND | ND | ND | ND |
| AD/PDR5− | 10c | 1.3s/2.5c | 2.5s/10c | 0.63s/1.25c | ND | ND | ND | ND |
| Fungal pathogen | ||||||||
| C. albicans ATCC 10261 | >20 | 20s | >10 | 5s/10c | 20 | 2 | 100/2.0 | 40/2.0 |
| C. glabrata CBS138 | >20 | >10 | 10s | 5s/10c | 10 | 2 | 100/17.5 | 20/17.5 |
| C. krusei B2399 | >20 | 20s | >10 | 10s | 6 | 1 | 100/17.5 | 40/1.0 |
| C. tropicalis IF0168 | 20c | 10s/20c | 5s | 2.5s/5c | 5 | 0.6 | 25/0.5 | 5/0.5 |
| C. neoformans ATCC 90121 | 10c | 5s/10c | 5c | 2.5s/10c | 3 | 1.5 | 25/0.5 | 10/2.0 |
a
MIC90, minimum concentration at which there is >90% growth inhibition compared to no-peptide control (s, fungistatic); MFC, peptide concentration at which no CFU was recovered (c, fungicidal).
b
Concentration of peptide that gives >98% cell death/time required to achieve this effect. ND, not determined.
c
The pHs in parentheses are those used in tests with fungal pathogens other than S. cerevisiae. A single pH value indicates that the same pH was used for both S. cerevisiae and the other fungal pathogens.