TABLE 6.

Effects of BM0 and BM2 on Pma1p and Na⁺,K⁺-ATPase kinetic properties^a

Strain	Vmax (μmol/min/mg of protein)	Km (mM) for ATP	IC50 (μM)
			Without ATP	With ATP
S. cerevisiae T48	1.2-1.3	1.4-2.0
With BM0 (3 μM)	0.62	1.9	2.5	2.5
With BM2 (0.8 μM)	0.73	3.3	0.5	0.5
C. albicans ATCC 10261	0.91-1.1	3.5-3.8
With BM0 (20 μM)	0.74	6.8	22	22
With BM2 (4 μM)	0.49	7.5	2.3	2.3
C. glabrata CBS138	0.22	1.8
With BM2 (3 μM)	0.10	1.7	1.3	1.8
C. krusei B2399	2.6	3.5
With BM2 (0.8 μM)	1.8	4.0	0.6	1.0
C. tropicalis IFO 0618	3.8	3.0
With BM2 (0.5 μM)	2.4	4.0	0.4	0.5
C. neoformans ATCC 90112	0.20	0.66
With BM2 (1.5 μM)	0.09	0.65	1.0	1.3
Dog Na+, K+-ATPase
With BM0	ND	ND	ND	62
With BM2	ND	ND	ND	25

^aAll assays were conducted at pH 7.0. ND, not determined.