TABLE 7.
BM2 chemosensitizes S. cerevisiae constructs and fungal pathogens to azoles
| Drug tested and species | Strain (hyperexpressed protein) | Concn used for chemosensitization/MIC
|
Fold chemosensitization by BM2 (for FLC or ITC MIC) | |
|---|---|---|---|---|
| BM2 (μM) | FLC or ITC (μg · ml−1) | |||
| FLC | ||||
| S. cerevisiae | T48 | 1.25/2.5 | 5/20 | 4 |
| AD/PDR5− | 1.25/2.5 | 0.5/1 | 2 | |
| AD/PDR5+ (Pdr5p) | 2.5/5 | 80/600 | 7.5 | |
| AD1-8u−a | 0.75/3 | 1/2 | 2 | |
| AD/PDR5 (Pdr5p)a | 1.75/3 | 320/40 | 8 | |
| AD/CaCDR1 (CaCdr1p)a | 1.5/2 | 80/320 | 4 | |
| AD/MDR1 (Mdr1p) | 1.5/2 | 3.75/60 | 16 | |
| AD/ERG11 (Erg11p) | 1.5/2 | 0.5/2 | 4 | |
| AD/CaCDR2 (CaCdr2p) | 2/3 | 20/80 | 4 | |
| AD/CgCDR1-1B (CgCdr1p)a | 1.75/3 | 40/320 | 8 | |
| C. albicans | ATCC 10261 | 10/20 | 0.25/>64 | >256 |
| FR2 | 5/20 | 2/>32 | >16 | |
| KB | 20/40 | 10/>160 | >16 | |
| C. glabrata | CBS138 | 5/10 | 20/>80 | >4 |
| 850821 | 10/40 | 80/160 | 2 | |
| 85090 | 5/40 | 2.5/>40 | >16 | |
| C. tropicalis | IF00618 | 1/3 | 1.25/>160 | 128 |
| C. krusei | B2399 | 10/20 | 20/>80 | >4 |
| IFO 0011 | 10/20 | 20/>80 | >4 | |
| 89.102 | 10/20 | 40/>80 | >2 | |
| C. dubliniensis | CD36 | 5/20 | 0.3/>20 | >60 |
| CD43 | 2.5/20 | 1.25/>20 | >16 | |
| C. parapsilosis | 90.493 | 10/40 | 2/8 | 4 |
| 425 | 40/40 | >32/>32 | Not detected | |
| ITC | ||||
| S. cerevisiae | AD1-8u− | 1.5/3 | 0.2/0.8 | 4 |
| AD/PDR5 | 1.75/3 | 10/>40 | >4 | |
| AD/CDR1 | 1.75/3 | 20/>40 | >2 | |
| AD/CgCDR1-1B | 1.75/4 | 20/>40 | >2 | |
| C. glabrata | CBS138 | 5/10 | 0.2/>0.8 | >4 |
| C. krusei | B2399 | 10/20 | 0.05/>0.8 | >16 |
| IFO 0011 | 10/20 | 0.05/0.8 | 16 | |
| 89.102 | 10/20 | 0.05/>0.8 | >16 | |
a
Incubated for 72 h instead of 48 h to allow full development of resistance phenotype.