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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: Mol Cell. 2017 Feb 16;65(4):659–670.e5. doi: 10.1016/j.molcel.2017.01.034

Figure 2. Maternally transmitted epigenetic silencing.

Figure 2

(A) Normalized sid-1 mRNA levels (relative to cpf-1) in single silenced and non-silenced parents and progeny (see Figure S2B for non-normalized data). Average ± SD of at least two technical replicates. (B) RNAi sensitivity of progeny (average of 62 per worm) from (n) F2 cross progeny described in (A) and their subsequent self-progeny fed dpy-11 RNAi. (C) RNAi sensitivity of progeny (average 68 per worm) from (n) F2 sid-1+/nDf32 hemizygous or sid-1+/sid-1+ cross progeny fed dpy-11 RNAi. (D) qRT-PCR measurement of sid-1 intron 4 and intron 6 expression in single worm adults relative to cpf-1 intron 5 expression. cDNA used for qRT-PCR was generated using a gene specific primer amplifying sid-1 and cpf-1. Average ± SD of at least two technical replicates is shown. See also Figure S2.