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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: Mol Cell. 2017 Feb 16;65(4):659–670.e5. doi: 10.1016/j.molcel.2017.01.034

Figure 6. Transgenerational somatic silencing.

Figure 6

(A) Progeny of wild-type or Psid-1::gfp larvae (two generations) or embryos (one generation) placed on dpy-11 RNAi food. (B–D) RNAi sensitivity of (n) worms hatched on dpy-11 RNAi food, scored as adults. To determine sid-1 genotype in (B), adults were fed act-5 RNAi (L1 arrest). (E) qRT-PCR analysis of sid-1 mRNA levels (normalized, relative to gpd-2/3) in young adults. Average ± SD of at least two technical replicates. (F) RNAi sensitivity of methyltransferase mutants after dpy-11 dsRNA exposure for one (n worms scored) or two generations (average of 116 progeny from (n) L4 larvae scored). See also Figure S6.