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. 2015 Feb 12;5:8427. doi: 10.1038/srep08427

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(a) DCs (10⁵) labeled with different nanoparticle preparations (10 μmol PFCE) were introduced to glass dishes containing CCL21-agarose spots and the number of cells entering the agarose spot was visualized by acquisition of microscope images. (b) DCs (10⁵) labeled with increasing PFCE concentrations of both basic and DPPE-enriched ¹⁹F nanoparticles were introduced to glass dishes containing CCL21-agarose spots and the number of cells entering the agarose spot was determined by acquiring and fusing the microscope images for all the fields of views (FOV) and counting all cells entering the corresponding spot. This experiment is representative of 3.