Figure 4. Effect of miR-612, miR-661 and miR-940 on RPE1 migration and proliferation.

(a) Motility graph: Normalised number of cells for the transwell assay. RPE1 cells were independently transfected with mimics of miR-661, miR-612 and miR-940. The number of cells that passed through the 5 µm holes after 18 hours were counted. Four independent experiments were conducted. The cell number was normalised based on the negative control cells, and all four experiments were pooled. P-values were calculated using the non-parametric two sided Mann-Whitney test. (b) Wound healing assay. Relative wound closure after a scratch was made in confluent cells transfected by mimics of the three microRNAs. The experiment was conducted on 10 hours with images taken at t₀, t₀+5h, t₀+7.5h, and t₀+10h. Each condition was present in triplicates. (c) Vinculin and phalloïdin immunostaining images of the cells transfected by mimics of the three microRNAs. The images were taken at the AxioImager®.