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. 2015 Feb 10;5:8220. doi: 10.1038/srep08220

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(A) Interaction of 1-HP with iron (FeCl₃) in absence or presence of EDTA, showing an immediate change yellow colored 1-HP in the presence of iron and absence of EDTA, and flocculation of the [1-HP-iron] complex after 24 h incubation. (B) λ_560 nmtitration curve of the [1-HP-iron] complex in presence of increasing concentrations of FeCl₃. (C) LC-MS analysis of the [1-HP-iron] flocculation product. (D) Deduced structures of the complexes formed by 1-HP and FeCl₃ based on LC-MS analysis, compared to the exact mass and structure of 1-HP.