Fig. 2.

TP73-AS1 knockdown inhibited HCC cell proliferation and downregulated HMGB1 signal pathway a TP73-AS1 knockdown was achieved by si-TP73-AS1 and the inhibitory efficiency was verified by real-time PCR. b MTT assays was performed to determine the proliferation of HCC cell lines, HCCLM3 and HepG2, in response to knocking down of TP73-AS1, compared with si-NC group. c BrdU was performed to determine the proliferation of HCC cell lines, HCCLM3 and HepG2, in response to knocking down of TP73-AS1, compared with si-NC group. d and e Colony formation assay was performed to determine the colony formative capacity of HCC cell lines, HCCLM3 and HepG2, in response to knocking down of TP73-AS1, compared with si-NC group. f and g The protein levels of HMGB1, RAGE and NF-κB were determined in si-TP73-AS1-transfected HCCLM3 and HepG2 cells. The data are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01