Fig. 5.

TP73-AS1 regulated HMGB1/RAGE expression and NF-κB targets cytokines levels through miR-200a a and b HepG2 and HCCLM3 cell lines were co-transfected with si-TP73-AS1 and miR-200a inhibitor. Then the protein levels of HMGB1, RAGE and NF-κB were determined by using Western blot assays. The gradation analysis of the Western blot films were performed using Image J software. c The levels of TNF-α, IL-6 and IL-1βin cultured media were measured by ELISA. The data are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 vs. inhibitor-NC + si-NC group, # P < 0.05, ## P < 0.01 vs. inhibitor-NC + si-TP73-AS1 group