Fig. 4.

Methylation age is affected by biological interventions. a Predicted age of 20-week-old liver samples [30], shown separately for males and females. Statistical test performed: t-test, p value of 0.58. b Age prediction in test samples from various biological intervention studies. X-axis shows the actual age and y-axis shows the predicted age, coloured by study. c Age prediction in normal female liver samples and samples which underwent an ovariectomy and were administered with either vehicle alone or testosterone [30]. Statistical test performed: Unpaired two-tailed t-test performed to assess the impact of ovariectomy, p value of 0.014. d Age prediction in diet perturbation study [29]. Liver samples from animals with following treatments were analysed: maternal high fat diet followed by adult high fat diet, maternal high fat diet followed by adult low fat diet, maternal low fat diet followed by adult high fat diet, and maternal low fat diet followed by adult low fat diet. Statistical test performed: two-way ANOVA performed, p values displayed where significant