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. 2017 Apr 12;17:74. doi: 10.1186/s12870-017-1018-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of sucrose starvation on metabolite levels (on a fresh weight basis) on 3 DAP B73 kernels (2012 season). All metabolites were measured using anion exchange chromatography in tandem with mass spectrometry as described by Lunn et al. [18]. Control kernels on continuous sucrose are indicated with closed squares. Kernels starved for 48 h (shaded area), then allowed to recover for 48 H on 150 mM sucrose are indicated with open squares. Means are calculated using 4 biological replicates, consisting of 3 plants each. Significance as indicated by asterisks was determined by the Student’s t test (P < 0.05)