Fig. 7.

RT-qPCR was performed on total RNA extracted from maize kernels cultured in vitro on MS complete agar ±150 mM sucrose. Open squares indicate kernels starved for sucrose, and closed squares indicate kernels subjected to sucrose starvation. Means are plotted based on 3 biological replicates. At −2 h the ears were removed from the plant. The vertical dashed line indicates when kernels were placed on medium