FIG. 3.

Induction of PGE₂ by C. pneumoniae. Monocytes were incubated with different concentrations of C. pneumoniae IFU for 6 h, washed, and cultured with or without TNF-α plus GM-CSF for 24 h. The 24-h supernatants were assayed for PGE₂ by ELISA (A) and from cultures treated with 1 μM indomethacin (B). The data are representative of three separate experiments from different donors.