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. 2017 Mar 21;114(14):E2826–E2835. doi: 10.1073/pnas.1613447114

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Fig. S1. — CMC determination for PtdIns(4,5)P2(di-C16). (A) The wavelength of the 1-pyrenecarboxaldehyde fluorescence maximum at varying PtdIns(4,5)P2(di-C16) concentrations was monitored under kinetic assay conditions (79) for excitation at 380 nm (89). The emission data were fit to fifth-order polynomials (curves shown) in GraphPad Prism to determine the position of the maxima. (B) The shift in emission wavelength with lipid concentration was fit to a Boltzmann sigmoid function in Prism. The inflection point, considered the CMC, occurs at 3.0 μM PtdIns(4,5)P2(di-C16).