Fig. S2.
Western blot analysis of three IDE variants showing approximately equal staining. A 10% SDS polyacrylamide gel was loaded with 0.25 µg of each IDE variant used in the study. Western blot analysis was performed, and the three bands were quantified as described in Materials and Methods. The IDEK898A,K899A,S901A mutant gave a value of 0.83 compared with WT IDE, and IDEY609F gave a value of 0.94. These values are within the limits of our experimental error and would not account for the differences found in subcellular fractionation studies.