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Marker proteins for COS-1 cell fractionation. Subcellular compartment marker proteins were visualized by Western blot analysis for the COS-1 cell fractionation shown in Fig. 5 A–C. The markers are Na+/K+ ATPase, plasma membrane; Rab-5, endosome; and EEA1, endosome. Results are shown as indicated for cells transfected with IDEwt, IDEK898A,K899A,S901A, or IDEY609F.
