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Immunofluorescence labeling of a control OM protein requires cell permeablization. CglC, a lipoprotein localized to the inner leaflet of the OM (19), was not detected following the immunofluorescence procedure used to detect TraA on live cells (Left; compare with Fig. 2B). CglC was detected when cells were permeabilized before immunofluorescence labeling (Right). Strains are DW1466 (cglC−) and DK8601 (cglC+). (Scale bar, 2 μm.)
