Fig. S4.

Reassignment of supergroup D into two recognition groups: D and F. Interactions between indicated TraA receptors were assessed in isogenic backgrounds by stimulation assays (Left). Panels with red borders show group D members, whereas the green borders show group F members. These results were combined with lipophilic dye transfer results (4) and summarized (Right). All group D members contain an A that corresponds to residue 205 in TraA^A96, whereas all group F members contain a P at this position. Alleles that were not analyzed here include receptors with identical variable domains (traA^A47 and traA^A96; traA^DK852 and traA^DK823) or very similar and compatible variable domains (traA^Mxx23 and traA^A96 contain four amino acid differences, whereas traA^DK836 and traA^DK854 contain three amino acid differences). White arrows indicate poor cross-talk between receptors. (Scale bar, 200 μm.)