Table S1.
Plasmids and strains used in this study
| Plasmids/strains | Relevant features* | Source |
| Plasmids | ||
| pDP22 | pSWU19 Mx8 attP cassette, PpilA, Kmr | (4) |
| pDP23 | pDP22 PpilA-traADK816, Kmr | (4) |
| pDP24 | pDP22 PpilA-traAA96, Kmr | (4) |
| pDP25 | pDP22 PpilA-traAMf, Kmr | (4) |
| pDP26 | pDP22 PpilA-traAPali, Kmr | (4) |
| pDP27 | pDP22 PpilA-traADK1622, Kmr | (4) |
| pDP28 | pBJ114 ΔtraA cassette, galK Kmr | (4) |
| pDP35 | pBJ114 ΔtraAB cassette, galK Kmr | This study |
| pDP36 | pDP22 PpilA-traBDK1622, Kmr | This study |
| pPC1 | pKSAT PpilA-SSOM-sfGFP, Smr | (14) |
| pPC2 | pKSAT PpilA-EYFP, Smr | This study |
| pPC3 | pDP22 PpilA-traADK1622 ΔSS, Kmr | This study |
| pPC4 | pDP22 PpilA-traABMf, Kmr | This study |
| pPC5 | pDP22 PpilA-traADK816traBDK1622, Kmr | This study |
| pPC6 | pDP22 PpilA-traADK805traBDK1622, Kmr | This study |
| pPC7 | pDP22 PpilA-traAMf205A96, Kmr | This study |
| pPC8 | pDP22 PpilA-traAA96205Mf, Kmr | This study |
| pPC9 | pDP22 PpilA-traAMf192A96, Kmr | This study |
| pPC10 | pDP22 PpilA-traAA96192Mf, Kmr | This study |
| pPC11 | pDP22 PpilA-traAMf192A96205Mf, Kmr | This study |
| pPC12 | pDP22 PpilA-traAA96192Mf205A96, Kmr | This study |
| pPC13 | pDP22 PpilA-traAMf192A96205Mf (A192G), Kmr | This study |
| pPC14 | pDP22 PpilA-traAMf192A96205Mf (V201M), Kmr | This study |
| pPC15 | pDP22 PpilA-traAMf192A96205Mf (A205P), Kmr | This study |
| pPC16 | pDP22 PpilA-traADK805, Kmr | This study |
| pPC17 | pDP22 PpilA-traADK816 (P205A), Kmr | This study |
| pPC18 | pDP22 PpilA-traAPali (A205P), Kmr | This study |
| pPC19 | pDP22 PpilA-traADK805 (A205P), Kmr | This study |
| pPC20 | pDP22 PpilA-traADK1622 (A205P), Kmr | This study |
| pPC21 | pDP22 PpilA-traAGVK897, Kmr | This study |
| pPC22 | pDP22 PpilA-traADK823, Kmr | This study |
| pPC23 | pDP22 PpilA-traADK854, Kmr | This study |
| pPC24 | pDP22 PpilA-traAMftraBDK1622, Kmr | This study |
| Strains | ||
| DH5α | E. coli cloning strain | Lab collection |
| DK1622 | A+S+, WT | (17) |
| DK8601 | A–S–, aglB1 (aglQ1) ΔpilA::tc, Tcr | (36) |
| DW1463 | A–S–, DK8601(pXW6, pDP21), Kmr, Tcr, Smr | (11) |
| DW1466 | A–S–, DK1622 Δtgl::tc ΔcglC (markerless), Tcr | (11) |
| DW1467 | A–S–, DK8601 ΔtraA (markerless), Tcr | (4) |
| DW1468 | A–S–, DW1467 (pDP23), Kmr, Tcr | (4) |
| DW1469 | A–S–, DW1467 (pDP24), Kmr, Tcr | (4) |
| DW1470 | A–S–, DW1467 (pDP25), Kmr, Tcr | (4) |
| DW1471 | A–S–, DW1467 (pDP26), Kmr, Tcr | (4) |
| DW1483 | A–S–, DK8601 ΔtraAB (markerless), Tcr | This study |
| DW2201 | A–S–, DK8601(pPC1, pDP21), Kmr, Tcr, Smr | (14) |
| DW2202 | A–S–, DK8601 (pDP27), Kmr, Tcr | This study |
| DW2203 | A–S–, DK8601 (pDP36), Kmr, Tcr | This study |
| DW2204 | A–S–, DK1467 (pPC3), Kmr, Tcr | This study |
| DW2205 | A–S–, DW1483 (pDP27), Kmr, Tcr | This study |
| DW2206 | A–S–, DW1483 (pPC4), Kmr, Tcr | This study |
| DW2207 | A–S–, DW2206 (pPC1), Kmr, Tcr, Smr | This study |
| DW2208 | A–S–, DW1483 (pPC5), Kmr, Tcr | This study |
| DW2209 | A–S–, DW1483 (pPC2, pPC6), Kmr, Tcr, Smr | This study |
| DW2210 | A–S–, DW1467 (pPC7), Kmr, Tcr | This study |
| DW2211 | A–S–, DW1467 (pPC8), Kmr, Tcr | This study |
| DW2212 | A–S–, DW1467 (pPC16), Kmr, Tcr | This study |
| DW2213 | A–S–, DW1467 (pPC17), Kmr, Tcr | This study |
| DW2214 | A–S–, DW1467 (pPC18), Kmr, Tcr | This study |
| DW2215 | A–S–, DW1467 (pPC19), Kmr, Tcr | This study |
| DW2216 | A–S–, DW1467 (pPC20), Kmr, Tcr | This study |
| DW2217 | A–S–, DW1467 (pPC21), Kmr, Tcr | This study |
| DW2218 | A–S–, DW1467 (pPC22), Kmr, Tcr | This study |
| DW2219 | A–S–, DW1467 (pPC23), Kmr, Tcr | This study |
| DW2220 | A–S–, DW1466 ΔtraA (markerless), Tcr | This study |
| DW2221 | A–S–, DW2220 (pDP23), Kmr, Tcr | This study |
| DW2222 | A–S–, DW2220 (pDP24), Kmr, Tcr | This study |
| DW2223 | A–S–, DW2220 (pDP25), Kmr, Tcr | This study |
| DW2224 | A–S–, DW2220 (pDP26), Kmr, Tcr | This study |
| DW2225 | A–S–, DW2220 (pPC7), Kmr, Tcr | This study |
| DW2226 | A–S–, DW2220 (pPC8), Kmr, Tcr | This study |
| DW2227 | A–S–, DW2220 (pPC9), Kmr, Tcr | This study |
| DW2228 | A–S–, DW2220 (pPC10), Kmr, Tcr | This study |
| DW2229 | A–S–, DW2220 (pPC11), Kmr, Tcr | This study |
| DW2230 | A–S–, DW2220 (pPC12), Kmr, Tcr | This study |
| DW2231 | A–S–, DW2220 (pPC13), Kmr, Tcr | This study |
| DW2232 | A–S–, DW2220 (pPC14), Kmr, Tcr | This study |
| DW2233 | A–S–, DW2220 (pPC15), Kmr, Tcr | This study |
| DW2234 | A–S–, DW2220 (pPC16), Kmr, Tcr | This study |
| DW2235 | A–S–, DW2220 (pPC17), Kmr, Tcr | This study |
| DW2236 | A–S–, DW2220 (pPC18), Kmr, Tcr | This study |
| DW2237 | A–S–, DW2220 (pPC19), Kmr, Tcr | This study |
| DW2238 | A–S–, DW2220 (pPC20), Kmr, Tcr | This study |
| DW2239 | A–S–, DW2220 (pPC21), Kmr, Tcr | This study |
| DW2240 | A–S–, DW2220 (pPC22), Kmr, Tcr | This study |
| DW2241 | A–S–, DW2220 (pPC23), Kmr, Tcr | This study |
| DW2242 | A–S–, DW1467 (pPC24), Kmr, Tcr | This study |
*
Fusion sites for chimera construction are highlighted in bold (superscript).