Table S2.
Primers used in this study
| Primer name | Sequence, 5′–3′* |
| pSWU19-EcoRI-PpilA-F | AGGAAACAGCTATGACCATGATTACGAATTCCGTCATGTTGGACGAGGT |
| pSWU19-XbaI-HindIII-TraA-R | TGCATGCCTGCAGGTCGACTCTAGAAAGCTTGAAGAGCTGCACGTTGAAG |
| Mf205A96-R | GTAACGCTGTTGGTGGTTCGCCAC |
| Mf205A96-F | GTGGCGAACCACCAACAGCGTTAC |
| A96205Mf-R | Same as Mf 205A96-R |
| A96205Mf-F | Same as Mf 205A96-F |
| Mf192A96-R | GTGGCCGGTTGATGACTTGGTAGGCCTGGCTGCGGTCGTAGAT |
| Mf192A96-F | GCCTACCAAGTCATCAACCGG |
| A96192Mf-R | GTGGCCGATTGATGACCTGATATCCTTGGCTGCGGTCGTAGATG |
| A96192Mf-F | GGATATCAGGTCATCAATCGGC |
| Mf192A96205Mf-R | Same as Mf 205A96-R |
| Mf192A96205Mf-F | Same as Mf 205A96-F |
| A96192Mf 205A96-R | Same as Mf 205A96-R |
| A96192Mf 205A96-F | Same as Mf 205A96-F |
| Mf192A96205Mf (A192G)-R | GTGGCCGGTTGATGACTTGGTATCCCTGGCTGCGGTCGTAGAT |
| Mf192A96205Mf (A192G)-F | GGATACCAAGTCATCAACCGGCCAC |
| Mf192A96205Mf (V201M)-R | TGGTTCGCCACGTCGCGAATCCGAGCATCGGTGGCCGGTTGATGAC |
| Mf192A96205Mf (V201M)-F | CTCGGATTCGCGACGTGG |
| Mf192A96205Mf (A205P)-R | GTAACGCTGTTGGTGGTTCGCCACGTTGGGAATCCGAGCACCGGTGG |
| Mf192A96205Mf (A205P)-F | Same as Mf 205A96-F |
| DK1622 (A→P)-R | TGGTCGTGCGCCAGGACGGGATTCCGAG |
| DK1622 (A→P)-F | CCACCGCAGCTCGGAATCCCGTCCTGGCGCACGA |
| DK816 (P→A)-R | GTCCGCCAGGTGGCCGCTCCTAGCTGGGGCG |
| DK816 (P→A)-F | AGCTAGGAGCGGCCACCTGGCGGACGACC |
| Pali (A→P)-R | GCCAAGTGGGCGCACCTAGCTCAGGCGG |
| Pali (A→P)-F | CCTGAGCTAGGTGCGCCCACTTGGCGGACGAGCA |
| DK805 (A→P)-R | GCCAGGTGGGCGCACCTAGCACTGGTGGG |
| DK805 (A→P)-F | CCAGTGCTAGGTGCGCCCACCTGGCGGTCGACCAAC |
| TraA-RBS-XbaI-F | GACGACTCTAGAGGAAACCAAGAATAGAAATAGAAAGGAGAATTAGTGGGAGATATCCCTCATTG |
| TraA-HindIII-R | GACGACAAGCTTGAAGAGCTGCACGTTGAAG |
| TraB-RBS- XbaI-F | GACGACTCTAGAGGATCGACGCCTATATAGACGGGAGATATAATGAAGCCCTCGCACCTG |
| TraB-HindIII-R | GACGACAAGCTTGGAGTTCTTCACCTCGGACTC |
| ΔtraA-Upstr-HindIII-F | CACACAAAGCTTGGGTGCTCTTCGAGGTGAT |
| ΔtraA-Upstr-EcoRI-PstI-R | CACACAGAATTCATCCTGCAGTGGTGAAGGAGCTACGGGA |
| ΔtraB-Dwnstr-PstI-F | CACACACTGCAGGAGGACAAGAAGCGCAAG |
| ΔtraB-Dwnstr-EcoRI-R | CACACAGAATTCCTCAATTCGCTGGACGTG |
| TraA ΔSS-RBS-XbaI-F | GACGACTCTAGAGGAAACCAAGAATAGAAATAGAAAGGAGAATTAATGCAGCCAGAGCCAGGCGAA |
| TraA (Mf)-RBS-XbaI-F | GACGACTCTAGAGGAAACCAAGAATAGAAATAGAAAGGAGAATTAATGGACGATATCCCTCATTCT |
| TraB (Mf)-HindIII-R | GACGACAAGCTTAGCTTGAGTCACTTCGCG |
| TraA-Upstr-R | ATATCTCCCATTAATTCTCCTTTCTATTTCTATTCTTGGTTTCC |
| TraA (different alleles)-F | AGAAAGGAGAATTAATGGGAGATATCCCTCATTGTTGCGG |
| TraA (different alleles)-R | ACGATGAGGAACAGGTGCGAGGGCTTCATCGAGCAGGGCGCCG |
| TraA (Mf)-R | ACGATGAGGAACAGGTGCGAGGGCTTCATCGGGCGGTGCGCCGGGTGCGGAGGAGCACGAA |
| TraB (DK1622)-F | AGCCCTCGCACCTGTTCC |
| TraB (DK1622)-HindIII-R | TGCATGCCTGCAGGTCGACTCTAGAAAGCTTGGAGTTCTTCACCTCGGACTC |
| pKSAT-HindIII-PpilA-F | ATGAGGATCGTTTCATATGAAGCTTCGTCATGTTGGACGAGGTC |
| pKSAT-PpilA-R | CTTGCTCACCATGGGGGTCCTCAGAGAAGG |
| EYFP-F | TCTGAGGACCCCCATGGTGAGCAAGGGCGAG |
| EYFP-EcoRI-R | TGGATCCCCCGGGCTGCAGGAATTCTTACTTGTACAGCTCGTCCATGCC |
*
Restriction sites are underlined; codons that are subjected to site-directed mutagenesis are in bold.