Fig. S5.

DRaCALA analysis of RelQ interactions with ³²P ATP, ³²P ppGpp, and ³²P pppGpp. (A) ppGpp, but not ATP, is displaced from RelQ by increasing concentrations of mRNA(MF). (B) Catalytically inactive RelQ mutant D82G (EF2671 locus numbering) efficiently binds pppGpp, but not ATP. Complex formation is monitored using 50 nM ³²P-labeled ATP, ppGpp, or pppGpp and increasing concentrations of RelQ. IC₅₀, EC₅₀, and the Hill coefficient (n_H) were calculated using the 4PL model (Hill equation). Error bars represent SDs of the mean. Each experiment was performed at least three times.