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Tetrameric structure of RelQ is not affected by substrates and/or mRNA. Here 10-μL samples of WT (black and brown traces) or D82G RelQ (EF2671 locus numbering, red traces) at a concentration of 18.5 μM were analyzed on a Superdex 200 Increase 5/150 GL analytical gel filtration column, either alone (A) or supplemented with 1 mM ATP and GDP (B), or with 60 μM mRNA(MF) along with 1 mM ATP and GDP (C).
