FIG. 3.

Pulmonary clearance and leukocyte recruitment in the lungs of C. neoformans-infected control mice and anti-TNF-α-treated mice. (A) CBA/J mice were infected intratracheally with 10⁴ CFU and treated with an anti-TNF-α MAb on day 0. Total lung CFU were enumerated at days 2, 3, 7, 21, and 35 postinfection. (B) Lung leukocytes were isolated from individual lungs as described in Materials and Methods. Total leukocytes were enumerated, and recruited leukocytes were calculated. (C-F) Samples of individual lung leukocyte suspensions (days 7, 14, 21, and 35 postinfection) were cytospun onto slides and stained with Wright-Giemsa stain for visual quantification of leukocyte subsets. Results are expressed as the mean number per lung ± SE. n = 8 mice/group/time point from two separate experiments. *, P < 0.05 for comparisons with C. neoformans-infected mice. Macs/Mono, macrophages/monocytes.