FIG. 4.

Cytokine production in lungs of anti-TNF-α-treated mice at day 14 post-C. neoformans infection. Leukocytes were isolated from whole lungs at day 14 postinfection and were cultured for 24 h without additional stimulation. Supernatants were collected and assayed by ELISA for IL-4, IL-5, IL-10, and IFN-γ levels. Results are expressed as means ± SEs. n = 8 mice/group from two separate experiments. *, P < 0.05 for comparisons with C. neoformans-infected mice. Cytokine levels in uninfected lungs are represented by the dashed line.