FIG. 6.

(A and B) Recruitment of lung leukocytes in neutrophil-depleted mice at days 7 (A) and 14 (B) post-C. neoformans infection. Mice were treated with an anti-Gr1 antibody on days 0, 2, 4, and 6. Samples of individual lung leukocyte suspensions were cytospun onto slides and stained with Wright-Giemsa stain for visual quantification of leukocyte subsets. Results are expressed as the mean number per lung ± SE. n = 8 mice/group/time point from two separate experiments. (C) CFU in the lungs of neutrophil-depleted mice were examined at days 7, 14, and 35 post-C. neoformans infection. Aliquots from lung digests were plated onto Sabouraud dextrose agar in 10-fold dilutions, and total numbers of CFU per lung were determined. Results are expressed as means ± SEs. n = 8 mice/group/time point from two separate experiments. *, P < 0.05 for comparisons with C. neoformans-infected control mice.