Skip to main content
. 2017 Mar 20;114(14):3720–3725. doi: 10.1073/pnas.1700678114

Fig. S3.

Fig. S3.

Effects of siRNAs to IRF2, FAM111A and RFC3 on replication of a RPXV SPI-1 deletion mutant. (A–C) Uninfected A549 cells were transfected with control (NT) siRNA or nonoverlapping siRNAs targeted to IRF2, FAM111A or RFC3. After 72 h, gene expression was determined by RT-qPCR. Data represents normalized fold change compared with actin mRNA. (D–F) A549 cells were transfected with control (NT) siRNA or nonoverlapping siRNAs targeted to IRF2, FAM111A, or RFC3 and infected with RPXV-ΔSPI1-GFP at a multiplicity of 0.001 PFU per cell. At 28 h of infection the cells were harvested to determine virus-encoded C11 gene expression by RT-qPCR.