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. 2017 Mar 21;114(14):E2965–E2974. doi: 10.1073/pnas.1618834114

Fig. 1.

Fig. 1.

UV sensitivity of RdDM and PTGS loss of function Arabidopsis plants. (A) (Top) Root growth assay. Seven-day-old WT, RdDM mutant plants (nrpd1, rdr2, dcl3, and ago4) were exposed to UV-C. Root growth was calculated relative to the corresponding untreated plants (±SD). Eight plants per replicate were used, and three independent biological replicates were performed; ddb2-3 was used as control as DNA repair deficient plants. (Bottom) CPDs removal assay. Histogram represents the amounts of CPDs 1 h after UV-C treatment (± SD). Intensity of each dot was quantified and normalized to that of CPDs at time 0 to calculate the remaining CPDs content after 1 h. Twenty plants per replicate were used, and experiments were duplicated; ddb2-3 was used as control as DNA repair deficient plants; t test *P < 0.01; **P < 0.05; ns, nonsignificant. (B) Same as A for PTGS-deficient plants (rdr6, dcl4, ago1, and ago2). (C) Genetic interactions between ddb2 and ago1. Seven-day-old single (ago1 and ddb2) and double (ddb2-ago1) mutant plants were exposed to UV-C and grown for 24 h either under light or in the dark. Because ddb2-2 is in the No ecotype, the control for double mutant plants is No/Col. Eight plants per replicate were used, and three independent biological replicates were performed; t test *P < 0.01; ns, nonsignificant compared with the corresponding single mutants for double mutant. (D) Genetic interactions between GGR and RdDM (nrpd1, rdr2, dcl3, ago4, and ddb2) and double mutant plants (ddb2-nrpd1, ddb2-rdr2, ddb2-dcl3, and ddb2-ago4). Because ddb2-2 is in the No ecotype, the control for double mutant plants is No/Col; t test *P < 0.01; **P < 0.05 compared with the corresponding WT plants. Eight plants per replicate were used, and three independent biological replicates were performed; ns, nonsignificant compared with the corresponding single mutants for double mutant. (E) Same as D for GGR and PTGS. Seven-day-old single WT (rdr6, dcl4, ago1, ago2, and ddb2) and double mutant plants (ddb2-rdr6, ddb2-dcl4, ddb2-ago1, and ddb2-ago2) were exposed to UV-C. Eight plants per replicate were used, and three independent biological replicates were performed; t test *P < 0.01; ns, nonsignificant compared with the corresponding single mutants for double mutants.