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. 2017 Mar 21;114(14):E2965–E2974. doi: 10.1073/pnas.1618834114

Fig. 4.

Fig. 4.

Intergenic 21-nt uviRNAs biogenesis. (A) Box plots representing the abundance of 21-nt uviRNAs at intergenic CPD-damaged regions in WT plants and in RNA POL IV-deficient plants (nrpd1) ± UV-C. *P < 0.01 calculated according to Wilcoxon matched-pairs signed rank test. (B) Box plots representing the abundance of 21-nt uviRNAs at intergenic CPD-damaged regions in WT plants (Col) and in rdr2, dcl2/4, dcl3/4, dcl2/3, and dcl2/3/4 plants. *P < 0.01 calculated according to Wilcoxon matched-pairs signed rank test; ns, nonsignificant. (C) Genetic interactions between RdDM and PTGS loss of function. Seven-day-old WT, single (ndpr1, rdr2, rdr6, dcl3, and dcl4) and double mutant plants (ndpr1-rdr6, ndpr1-dcl4, rdr2-dcl4, and rdr6-dcl3) were exposed to UV-C; t test *P < 0.01; **P < 0.05; ns, nonsignificant. (D) Genetic interactions between DCLs loss of function. Seven-day-old WT, single (dcl2, dcl3, and dcl4), double (dcl2/3, dcl2/4, and dcl3/4), and triple mutant plants (dcl2/3/4) were exposed to UV-C; t test *P < 0.01; ns, nonsignificant. (E) Box plots representing the abundance of 26- to 30-nt RNAs at intergenic CPD-damaged regions in WT plants and in RNA POL IV-deficient plants (nrpd1) ± UV-C. *P < 0.01 calculated according to Wilcoxon matched-pairs signed rank test. (F) Same as E for 26-nt RNAs. Shown is graphical representation of consensus ribonucleotide sequences of 26-nt RNAs mapping at intergenic CPD-damaged regions. *P = 0.0164 calculated according to Wilcoxon matched-pairs signed rank test. (G) Same as F for 27-nt RNAs **P < 0.01.