Fig. S1.

Genetic interactions of RdDM and PTGS with DR and TCR. (A) Genetic interactions between phrI, ago1, and ago4. Ten-day-old WT, single (ago1, ago4, and phrI) and double mutant plants (phrI-ago1 and phrI-ago4) were irradiated with UV-C (1,500 J/m², 3,000 J/m²) three times in a row every 2 d. (Left) Histogram representing the percentage of bleached plants. At least 20 plants were used per replicate, and this experiment was duplicated; t test *P < 0.01 compared with single mutant plants; ns, nonsignificant. (Right) Histogram representing the average number of leaves (±SD); t test *P < 0.01 compared with untreated plants; ns, nonsignificant. (B) Pictures showing the phenotype of Col, phrI, ago1, ago4, phrI-ago1, and phrI-ago4 plants 1 wk after the last UV-C exposure (3,000 J/m²; scale bar,  2 cm). (C) Genetic interactions between csa, nrpd1, rdr2, ago1, and ago4. Seven-day-old WT, single (csa, nrpd1, rdr2, ago1, and ago4) and double mutant plants (csa-nrpd1, csa-rdr2, csa-ago1, and csa-ago4) were exposed to 600 J/m² of UV-C and grown for 24 h either under light or in the dark. Eight plants per replicate were used, and three independent biological replicates were performed; t test *P < 0.01; ns, nonsignificant.