Fig. S5.

Normal chemokine-induced actin polymerization in naïve EVL/VASP dKO T cells, and normal chemokinesis and chemotaxis of EVL/VASP dKO activated T cells. (A) Time-course analysis of actin polymerization in WT and dKO naïve T cells in response to 100 ng/mL CCL21 stimulation (WT vs. dKO curve comparison not significant), measured by flow cytometry quantification of fluorescent phalloidin staining. (B) Activated T-cell migration across 5-μm pore Transwell chambers, either in the absence of chemokine or with CCL21 (1 μg/mL) both in the upper and lower wells (chemokinesis), or with CCL21 (1 μg/mL) only in the lower well (chemotaxis). Data are the mean of three independent experiments; error bars are SEM. Statistics in A are two-way ANOVA, statistics in B are paired t tests. ns, not significant.