Alternative activation of macrophages in BALB/c mice after an F. hepatica infection. Expression levels of β-actin, Fizz1, Arg1, Ym1, and iNOS were analyzed by RT-PCR in adherent peritoneal exudate cells isolated from mice prior to infection (day 0) and at 1, 7, 14, and 21 days postinfection (d.p.i.). The data shown are from a single mouse and are representative of the findings from four mice examined at each time point (the expression of each gene was similar in each mouse).