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. 2005 Jan;73(1):166–173. doi: 10.1128/IAI.73.1.166-173.2005

FIG. 2.

FIG. 2.

The PI fraction of F. hepatica ES products contains TPx and induces Th2 responses when intraperitoneally injected into BALB/c mice. (a) Antigen-specific production of IL-4 and IL-5 from spleen cells of BALB/c mice injected with ES products or fractions of ES products, termed PI and PII. Spleen cell cultures were stimulated with 20 μg of the relevant injected antigen—ES product, PI, or PII—per ml and PMA-anti-CD3 as a positive control. The data are presented as mean ± the standard error (SE) for four mice. (b) ES product, PI, and PII separated on a SDS-12.5% gel stained with Coomassie blue. (c) ES product, PI, and PII fractions separated by SDS-PAGE and analyzed by Western blots probed with antisera prepared against recombinant TPx.