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. 2017 Mar 20;114(14):E2955–E2964. doi: 10.1073/pnas.1616363114

Fig. S3.

Fig. S3.

Rescue of myrlysin and lyspersin in KD neurons (Fig. 3). (A) DIV 7 neurons expressing LAMP1-RFP (red channel) and myrlysin-shRNA/GFP (green channel) in the absence (Top) or presence (Bottom) of shRNA-resistant myrlysin tagged with the myc epitope (blue channel, Inset). (B) Neurons expressing LAMP1-RFP (red channel) and lyspersin-shRNA/GFP (green channel) in the absence (Top) or presence (Bottom) of shRNA-resistant lyspersin tagged with the myc epitope (blue channel, Inset). Arrows point to the AIS and arrowheads to the axon. Quantification of LAMP1-RFP intensity ratios is shown (Right) as mean ± SD from 20 neurons per condition. Statistical significance for all groups was calculated by one-way ANOVA followed by Dunnett’s test (*P < 0.01, myrlysin-shRNA or lyspersin-shRNA vs. control and rescue).