Fig. S5.
Comparison of (1 – PRE) of D (yellow) or E (red) of the single-ring mutants with simulated binding curves for the one-site model. For the PRE data, 20 µM mutant channels were titrated with 0, 4, 6, 10, 15, 20, 25, 30, and 40 µM Mn2+ under our NMR titration condition. The binding curve was plotted using Eq. 1 for a fixed channel concentration (20 µM) at Kd values of 1, 10, 30, 100, and 300 μM.